The maximum capacity, qexp (mg g−1), obtained in a column system for a given feed concentration and flow rate could be calculated from the experimental data, i.e. area under breakthrough curve [51]. The complexity of mathematical calculation in the determination of adsorbent capacity by integrating total area below the breakthrough curve could be solved by different commercial software. An attractive alternative is a simplified modeling approach used for the prediction of dynamic behavior of the column. Multi-parameter fitting of the model to experimental points gives results ( Table 10) which appropriately describe adsorbent performances by using Bohart–Adams, Yoon–Nelson, Thomas and Modified dose–response ( Eqs. S8–S11). These models consider that NHS-SS-Biotin process limiting step is controlled by adsorption kinetics and can be applied only to one-component system, otherwise only results of experimental methodology are recombinant DNA molecules relevant.

Table 10.

Bohart–Adams, Thomas, Yoon–Nelson and Modified dose–response model fitting for As(V) adsorption by ER/DETA/FO/FD (CAs(V) = 0.33 mg g−1; t = 25 °C; pH = 5).Qcm3 min−10.512EBCTmin7.943.941.96Bohart–Adams modelkBAdm3 mg−1 min−10.08180.1770.547qomg g−122.918.89.65R20.990.990.99Thomas modelkthdm3 min−1 mg−10.1070.2540.767qemg g−123.819.39.75R20.990.990.99Yoon–Nelson modelkYNmin−10.03210.07630.232θmin26910927.6R20.990.990.99Modified dose–response modela4.224.313.88qomg g−121.416.87.90R20.890.900.93Full-size tableTable optionsView in workspaceDownload as CSV